Chromatographic enantioresolution and stereochemical characterization of synthetic cannabinoid receptor agonists with Whelk-O®1 chiral stationary phases under mass spectrometry compatible reversed-phase conditions: A study case with seized samples

<h2>Highlights</h2><ul><li>•Synthetic <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/cannabinoid-receptor-agonist" target="_blank">cannabinoid receptor agonists</a> were enantioseparated with Whelk-O®1 CSPs.</li><li>•Efficient enantioseparations were obtained under reversed-phase conditions.</li><li>•The developed HPLC method was compatible to MS detection.</li><li>•The <a href="https://www.sciencedirect.com/topics/chemistry/chirality" target="_blank">ICCA</a> concept was useful to appraise the <a href="https://www.sciencedirect.com/topics/chemistry/enantiopurity" target="_blank">enantiopurity</a> of the studied compounds.</li><li>•Coupling <a href="https://www.sciencedirect.com/topics/chemistry/electronic-circular-dichroism" target="_blank">ECD</a> analysis to <i>in silico</i> simulations allowed to establish the <a href="https://www.sciencedirect.com/topics/chemistry/enantiomer" target="_blank">EEO</a>.</li></ul><p></p>