Determinación de parentesco en alpacas (Vicugna pacos) por medio del análisis de ADN microsatélite

<p>Ten polymorphic microsatellites for alpacas and llamas were used to evaluate paternityin 47 alpacas (18 crias, 18 mothers and 11 fathers) registered at IVITA-Maranganí ResearchStation, Canchis Province (Cusco-Peru). Analysis was carried out using twomethodologies: Automatic Sequencer (ABI 377 DNA sequencers®) and silver stainingtechniques. Microsatellites were amplified in three multiple and ten single PCR reactions.The number of alleles varied between 4 and 20. The allelic frequencies and the exclusionprobability were calculated using Cervus 2.0. All loci, except for two, were within therange published elsewhere. The accumulated exclusion probability for the ten loci was0.9999. For each multiplex reaction the accumulated exclusion probability was more than0.90. Both methodologies yielded the same results. The results confirmed paternity in 18cases of parent-cria pairs, however in 22% of cases (n=4) were identified alternativeparents than those indicated in farm records</p>