A microparticle assembly

A microarray, comprising a plurality of bead constructs disposed thereon, wherein the bead constructs comprise: (a) a bead; (b) a fluorophore molecule, wherein the fluorophore molecule comprises at least two subunits connected to each other by at least one internal linker that provides conformational freedom to the at least two subunits, and wherein the optical signal emitted by the flurophore changes when reducing the conformational freedom of the at least two subunits to each other; (c) a first linker molecule connected to the bead and to one of the subunits of the flurophore molecule; (d) a second linker molecule connected to another subunit of the fluorophore molecule; and (e) a ligand molecule connected to the second linker.